Information about Hosting Offer
Hosting Offer: IMol Polish Academy of Sciences
Information
| Role | Hosting Offer |
|---|---|
| Organisation | IMol Polish Academy of Sciences |
| Organisation type | Public |
| City | Warsaw |
| HR Excellence in Research Award from the European Commission | no |
| National evaluation category | not evaluated |
| Faculty / Dept. / Division / Laboratory / Research group | Laboratory of RNA Biology |
| Website | https://www.imol.institute/magda-konarska |
| Thematic area/field | NZ – Life Sciences / NZ1 Molecular biology, structural biology, biotechnology |
| Key words | spliceosome, catalytic center, snRNA, catalysis |
| Short summary of activities and expertise | IMol Polish Academy of Sciences is a newly created scientific institution devoted to basic sciences that combines expertise from different research fields following the highest international standards. Its mission is to serve as an important nucleation point of excellence on the scientific map of Poland and to promote creativity, productivity, innovation, and collaboration. IMol is led by Professors Agnieszka Chacińska and Magda Konarska, advised by the International Scientific Board, and acts in partnership with the University Medical Center Göttingen. At present, it consists of seven research groups. IMol provides its scientists with the best working conditions to grow and implement their ambitious, scientific goals. For more information on IMol, go to: https://www.imol.institute/. The applicant will be hosted by the LABORATORY OF RNA BIOLOGY, headed by Prof. Magda Konarska. The Konarska Lab studies the architecture and function of yeast S. cerevisiae spliceosome to explore dynamic rearrangements of the catalytic core that facilitate the repositioning of substrates for the second step of splicing. A detailed understanding of the yeast spliceosome function allows for mechanistic predictions concerning splicing in all eukaryotes and helps to formulate rules governing the function of other complex macromolecular machines. |
| Role and profile of key persons (incl. supervisor) | Prof. Magda Konarska is deputy director of ReMedy IRAP and deputy director for science of the IMol Polish Academy of Sciences. She received a PhD from the Institute of Biochemistry and Biophysics PAS, followed by post-doc and later research associate positions with Prof. Phillip A. Sharp at the Center for Cancer Research, MIT, Cambridge, USA. Subsequently, Prof. Konarska established the Laboratory of Molecular Biology and Biochemistry at the Rockefeller University, New York. In 2015 she opened a Laboratory of RNA Biology at CeNT UW, which in 2021 was moved to IMol. She is a corresponding member of PAS, member of EMBO, and Academia Europaea. |
| Previous and current involvement in research and training programmes | Prof. Konarska carried out multiple grants awarded from various national (National Science Center, Foundation for Polish Science) and international (NIH, NIGMS), funding agencies. She is currently conducting two research projects: one funded by NCN - 1) OPUS 20 entitled “Structure-function analysis of the catalytic center of the spliceosome” and one funded by FNP - 2) “ReMedy, Regenerative Mechanisms for Health”. |
| Relevant publications and/or research/innovation products | 1) Eysmont K, Matylla-Kulińska K, Jaskulska A, Magnus M, Konarska MM (2019) Rearrangements within the U6 snRNA core during the transition between the two catalytic steps of splicing. Mol. Cell 75 (3), 538-548.e3, doi: 10.1016/j.molcel.2019.05.018. 2) Kurtovic-Kozaric A, Przychodzen B, Singh J, Konarska MM, Clemente MJ, Otrock ZK, Nakashima M, Hsi ED, Yoshida K, Shiraishi Y, Chiba K, Tanaka H, Miyano S, Ogawa S, Boultwood J, Makishima H, Maciejewski JP, Padgett RA (2015) PRPF8 defects cause missplicing in myeloid malignancies. Leukemia. 2015 Jan;29(1):126-36. doi: 10.1038/leu.2014.144. 3) Query CC, Konarska MM (2013). Structural biology: Spliceosome's core exposed. Nature. Jan 31;493(7434):615-6. doi: 10.1038/nature11857. 4) Query CC, Konarska MM (2012). CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome. RNA, 18(5):1001-13. doi: 10.1261/rna.029421.111. |
| Key research facilities, infrastructure, and equipment | IMol is a newly established institute located in the Flanders Business Park C building. The research groups are fully equipped by the ReMedy program and scientific grants of the National Science Center and the European Molecular Biology Organization. Recently the Institute has bought new, common equipment including: 1. Synergy Neo2 (a robust plate reader that enables a broad array of absorbance, luminescent and fluorescent readouts), 2. LightCycler 480 (a real-time PCR device that supports mono- or multicolor applications), 3. Amersham ImageQuant 800 (a highly sensitive and robust charge-coupled device, CCD, imagers for capturing high-quality images in life science applications), 4. BioComp gradient station (a unique system for ultra-centrifugation gradient forming, fractionation, and analysis), 5. Bioscreen C Pro (microplate reader designed for laboratories performing continuous monitoring of cell growth under a controlled set of conditions), 6. 10x Chromium Single Cell Controller (a specialized instrument for streamlined sample processing and barcoding during the preparation of single-cell sequencing libraries), 7. Confocal microscope ZEISS LSM 900 (a high-class confocal platform for 2D and 3D sample structure topography analysis), 8. InvivO2 Hypoxia Workstation (a platform for cell and tissue cultures maintaining a physiological, low level of oxygen in "in vivo" conditions), 9. Novocyte 2000R (a high-performance benchtop flow cytometer for cell analysis with enhanced sensitivity and resolution), and 10. Amersham Typhoon IP (laser-scanner platform for versatile imaging and precise quantitation of nucleic acids and proteins). Scientists at IMol have access to the Proteomics Core Facility dedicated to the measurement and analysis of peptides and proteins involved in biological processes and structures. Currently, it is equipped with a nanoLC Dionex Ultimate 3000 coupled with Q-Exactive HF-X mass spectrometer and UPLC-coupled Q-Exactive UHMR. |
| Hosting arrangements | IMol is a newly established institute located in the Flanders Business Park C building. The research groups are fully equipped by the ReMedy program and scientific grants of the National Science Center and the European Molecular Biology Organization. Recently the Institute has bought new, common equipment including: 1. Synergy Neo2 (a robust plate reader that enables a broad array of absorbance, luminescent and fluorescent readouts), 2. LightCycler 480 (a real-time PCR device that supports mono- or multicolor applications), 3. Amersham ImageQuant 800 (a highly sensitive and robust charge-coupled device, CCD, imagers for capturing high-quality images in life science applications), 4. BioComp gradient station (a unique system for ultra-centrifugation gradient forming, fractionation, and analysis), 5. Bioscreen C Pro (microplate reader designed for laboratories performing continuous monitoring of cell growth under a controlled set of conditions), 6. 10x Chromium Single Cell Controller (a specialized instrument for streamlined sample processing and barcoding during the preparation of single-cell sequencing libraries), 7. Confocal microscope ZEISS LSM 900 (a high-class confocal platform for 2D and 3D sample structure topography analysis), 8. InvivO2 Hypoxia Workstation (a platform for cell and tissue cultures maintaining a physiological, low level of oxygen in "in vivo" conditions), 9. Novocyte 2000R (a high-performance benchtop flow cytometer for cell analysis with enhanced sensitivity and resolution), and 10. Amersham Typhoon IP (laser-scanner platform for versatile imaging and precise quantitation of nucleic acids and proteins). Scientists at IMol have access to the Proteomics Core Facility dedicated to the measurement and analysis of peptides and proteins involved in biological processes and structures. Currently, it is equipped with a nanoLC Dionex Ultimate 3000 coupled with Q-Exactive HF-X mass spectrometer and UPLC-coupled Q-Exactive UHMR. |
| Key research facilities, infrastructure, and equipment | IMol administration is highly efficient and motivated, English-speaking and experienced in helping foreigners. It provides scientists with extensive assistance in recruitment process, grant applications and reports, purchases, applying for residence permits, and others. Moreover, IMol scientists are supported on a daily basis by lab managers. Formation of strong inter-institutional networks of IMol scientists is facilitated by two seminar series: IMol Science Club with external speakers and IMol Colloquia, focused on presentations by IMol PhD students, postdocs, and group leaders. The programs of both seminar series are available on the web page of IMol. |
| Intersectoral secondments options | The intersectoral secondment will be discussed between the fellow and the supervisor and adjusted to the interests and career goals of the candidate. |
| Other benefits | Private health care, Full technical, administrative and organizational support from professional English-speaking personnel, Participation in courses, scientific training, support from peers, and academic mentoring. |
| Candidate sought | The ideal candidate should be thoughtful, creative, independent, have a strong interest in the mechanism of pre-mRNA splicing, RNA manipulations, and basic techniques of molecular biology. Some background in yeast genetics and/or biochemistry will be beneficial. |
| Have you hosted POLONEZ Fellows or MSCA IF Fellows before? | no |
| Candidatures deadline | 2022-11-15 |
Contact | |
| First name | Michał |
| Last name | Wrzesiński |
| projekty@imol.institute | |
| Phone number | |
